The effect of plant growth regulators on in vitro growth of hazelnut and Taxol production in the callus
Message:
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
In this research, the effects of type and concentration of plant growth regulators (2,4-D, NAA, Kin, BAP, IBA, TDZ) on callus induction from different explants, in vitro micropropagation of hazelnut via single node explants and also amount of taxol in callus were investigated. For this, seed, leaf and stem explants surface sterilized with different treatment and cultured on MS medium containing 2,4-D or NAA (1, 2 and 4 mg/l) in combination with Kinetin and BAP (0.5 and 1 mg/l). In addition, in order to in vitro micropropagation, single node explants were prepared and cultured on MS medium containing different levels of plant growth regulators. The results indicated that the percentage of callus induction from leaf explants was significantly (p≤ 0.01) higher than that of seed and stem. The highest percentage of callus induction and fresh weight of callus was observed on MS medium containing 2 mg/l 2,4-D and 0.5 mg/l BAP. The highest percentage of root induction in single node explants of hazelnut was observed on MS medium containing 2 mg/l NAA and 0.5 mg/l Kin and the MS medium containing 2 or 4 mg/l NAA and 0.5 mg/l Kin. The highest number of leaves was obtained on MS medium containing BAP and 0.05 mg/l IBA and MS medium containing 2.5 mg/l TDZ and 0.05 mg/l IBA. The highest content of taxol was observed in the calli derived from seed and leaf explants on MS medium supplemented with 1 mg/l 2,4-D and 0.5 mg/l BAP.
Language:
Persian
Published:
Journal of Plant Research, Volume:30 Issue: 3, 2018
Pages:
550 to 566
magiran.com/p1797486  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 990,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
دسترسی سراسری کاربران دانشگاه پیام نور!
اعضای هیئت علمی و دانشجویان دانشگاه پیام نور در سراسر کشور، در صورت ثبت نام با ایمیل دانشگاهی، تا پایان فروردین ماه 1403 به مقالات سایت دسترسی خواهند داشت!
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 50 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!