Application Effects of Bacillus subtilis and Salicylic Acid on the Tomato Plants Against Fusarium oxysporoum f.sp. lycopersici and Meloidogyne javanica

In this study, direct application effects of antagonistic bacteria Bacillus subtilis and Salicylic acid on Fusarium growth Fusarium oxysporoum f.sp. lycopersici examined in vitro, in two formats, contrast test and volatile materials test. Effect of different concentrations of salicylic acid (2-8 mM) on Fusarium growth has tested as a manner mixing with medium. In greenhouse tests induction effects was resuled from application of Salicylic acid and Bacillus subtilison on activity of PAL as one of the most important enzymes in plant defense tomato which infected with Fusarium wilt and Meloidogyne javanica also it was investigated when the two diseases interact with each other. According to the results all Salicylic acid concentrations completely prevented growth of the fungui. In contrast tests and volatile, antagonist bacteria significantly controlled Fusarium that respectively was 34.8 and 35 %. Evaluation the activity PAL indicated that all treatments including bacteria and Salicylic acid effectively increased level of enzyme activity compared with control and infected plants with fungi and nematodes and highest enzyme activity was in plant treated with pathogens and B. subtilis and salicylic acid on fifth days after inoculation.