Assesment of Salinity Tolerance in Some Canola Cultivars using Morphophysiologic Traites and ISSR Markers

Canola (Brassica napus L.) is one of the most important world's oilseed crops. The salin soils and salinity is the most important environmental stress factor that affects production of rapeseed. In order to study salinity resistance in canola cultivars at seedling stage based on morphophisiyological traits and assessing relation of these traits with ISSR markers, 15 canola cultivars in two salinity stress levels (150 mM and 250 mM) and non stress condition (control) were studied at greenhouse condition as hydroponic culture method. In this study, salinity stress caused decreasing of all evaluated morphophisiyological traits, except Na to K ratio and Na content. Results of mean comparisons and classification by cluster analysis of cultivars in different conditions showed that the SLMO46 and PF were better than other cultivars in all traits,was superior for average of most traits. So, the Hyolla60 and Licord cultivars in all traits had lower amounts. In Molecular analysis using 11 ISSR primers, 45 polymorphic bands produced in studied canola cultivars. Means of Polymorphic Information Content and Marker Index for all primers were obtained 0.282 and 1.108, respectively. In cluster analysis using ISSR data and Nei`s genetic distanceand UPGMA method, the canola cuhtivars classified in three clusters. The minimum genetics distance obtained between Zarfam and Jevel cultivars (0.079) and the maximum distance observed between Quantum and Hyola60 whit SLMO46 cultivars (0.32).