Histological and histomorphometrical study of intestinal bulb in Shirbot, Barbus grypus

Barbus grypus is native fishes in Khuzestan province which its species with a wide distribution in the rivers that considered fisheries research in province. The fish have no stomach, space of intestine is narrow to store of food, for this reason anterior intestine dilated bowel and called intestinal bulb. Study of histological structure of intestinal bulb in fishes can help to nutrition and food needs so that we can extend of this species. In this study, 10 adults Barbus grypus of both sexes with average weigh 354.25 ± 60.52 g and average length 36.25 ±4.16 cm was chosen, and after easy drawing, samples were removed from intestinal bulb. The 0.5cm were fixed by Bouin´s fixative soluble and 5-6µ thickness sections were made by paraffin embedding method and were stained by H&E and PAS. The histomorphometrical studies were done using digital Dino-Lite lens and Dino-capture1 software. The results showed that the number of goblet cells in the intestine increased toward the end of the intestinal bulb. The intestinal epithelium height in the anterior part of the intestinal bulb was higher than other parts. Muscular thickness was different in different parts of the intestinal bulb, the middle part of intestinal bulb has thickest muscle between other parts of intestinal bulb. The results of this research indicate that there is some analog histological structure of the intestinal bulb in Barbus grypus to compare of other same family: from wall diameter and intestinal epithelium height and diameter of mucosa.