Determination of Sensitivity Level of Hypocotyl Explants to Paromomycin Antibiotic and Optimization of Rooting in Transgenic Medicinal Plant Papaver somniferum
Author(s):
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Poppy (Papaver somniferum) is the most important commercial source of analgesic drugs and of compounds related to them. Because of the excellent medicinal prop- erties of poppy, finding genotypes with changed alkaloid content through breeding or by producing transgenic plants in tissue culture has attracted great interest. The present research was conducted to optimize rooting in somatic embryos of trans- genic poppy plants. Sensitivity of hypocotyl explants to the antibiotic paromomycin was first determined in kill-curve experiments. Transformation of the hypocotyl explants was carried out using the GV3101 strain of Agrobacterium tumefaciens having the vector with the NPT II gene. The explants were then transferred to MS- based medium containing the 2, 4-D plant growth regulator (PGR) at 1 mg/L, 10 mM MES, and paromomycinat 15 mg/L. After the somatic embryos were pro- duced, a factorial experiment was conducted in the rooting stage with two factors including medium (MS and 1/2 MS) and PGR (500 μM IBA PGR and no PGR). Also, confirmation of derived transgenic plantlets was confirmed by PCR tech- nique. Results of ANOVA and comparison of the means indicated the highest root- ing percentage happened in the 1/2 MS medium containing the IBA.
Keywords:
Language:
English
Published:
Journal of Applied Biotechnology Reports, Volume:4 Issue: 3, Summer 2017
Pages:
651 to 655
magiran.com/p1807213
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
دسترسی سراسری کاربران دانشگاه پیام نور!
اعضای هیئت علمی و دانشجویان دانشگاه پیام نور در سراسر کشور، در صورت ثبت نام با ایمیل دانشگاهی، تا پایان فروردین ماه 1403 به مقالات سایت دسترسی خواهند داشت!
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!