Effect of different solvents on extraction of phenolic and flavonoid compounds and antioxidant activity of Citrullus colocynthis
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Plants are rich sources of phenolic compounds which are important sources of antioxidants. Due to the antioxidant properties of Citrullus colocynthis, optimizing the extraction of phenolic compounds and antioxidant activity is important. The objective of this research was to study effect of different solvents (methanol, acetone, ethyl acetate, hexane and chloroform) in the extraction efficiency of phenolic and flavonoid compounds from different parts (leaf, seed, fruit pulp and fruit peel) of C. colocynthis. Phenolic and flavonoid contents were measured by Folin–Ciocalteu and Aluminum chloride methods respectively. Antioxidant activity of extracts was determined using Diphenyl Picrylhydrazyl (DPPH) and Hydrogen peroxide (H2O2) and (FRAP) assays. According to Tukey test at 5% level, results showed the solvent used for extraction has significant effect on the phenolic, flavonoid content and antioxidant activity. The methanol extract of C. colocynthis leaf, showed the highest phenolic content (27.01 mg/g DW). The extraction of fruit pulp with chloroform yielded the least amount of phenolic compounds (1.11 mg/g DW). Significant amount of phenolic and flavonoid content was observed in leaf methanol and acetone extract. Methanol and acetone extract had highest antioxidant activity in all antioxidant assays. Overall, the results show that methanol and acetone, are more effective solvents for phenolic compounds extraction. There is a positive correlation between the amount of phenolic compounds and flavonoids with antioxidant activity. Base on the results of this study, C. colocynthis leaves due to the high levels of phenolic compounds, suggest as supplement rich in natural antioxidants.
Language:
Persian
Published:
Food Science and Technology, Volume:15 Issue: 1, 2018
Pages:
313 to 320
magiran.com/p1811441  
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