Evaluating the efficacy of HI and ELISA tests and comparing them with CBC in challenge with AI (H9N2) and IBD Viruses in ostrich

In this study, the Hemagglutinin inhibition (HI) and ELISA testes were checked and comprised with CBC in challenge with Infectious Bursal Disease (IBD) and Avian Influenza (AI) sub type H9N2 viruses in ostrich. Eight ostrich chicks one months were prepared and divided randomly in two equal groups. Group 1 received IBD virus and group 2 received AI (H9N2) virus. Then on 2 days prior to challenge and 11 and 21 days post-challenge sera samples and on 2 days prior to challenge and 3, 11 and 21 days post-challenge blood smears were prepared from group 1 and on 2 days prior to challenge and 7 days post-challenge sera samples and on 2 days prior to challenge and 7 and 18 days post-challenge blood smears were prepared from group 2. The results were evaluated and analyzed by Sigma Stat software. In group 1, antibody titer significantly increased on 21 day post-challenge compared with 2 day prior to challenge and 11 day post-challenge but CBC didn’t show a significant change in after and before the challenges. In group 2, antibody titer significantly increased on 7 day post-challenge compared with 2 day prior to challenge but CBC didn’t show a significant change in before and after the challenges. The present study show that Infectious Bursal Disease virus infected the ostrich but didn’t any effects on CBC. HI and ELISA testes respectively are useful technique for detection of IBD and AI antibody after challenges in ostrich.