Molecular and Phylogenetic Analysis of Human Hydatid Cysts

Hydatidosis is one of the most common diseases in humans and livestocks caused by the larval stage of Echinococcus granulosus. The hydatid cyst are occurred in different organs of humans as the intermediate hosts. The economic burden of the disease in Iran is very high.
The current retrospective study was conducted from 2007 to the beginning of 2015 in hospitals and health centers of Bojnourd, Iran, on 90 people who underwent surgery for hydatid cyst. In 60 subjects protoscolex was observed in the germ layer tissue of hydatid cyst studied using DNA extraction and molecular phylogenetic methods.
A total of 90 patients were enrolled in the current study and followed up for 6 years. Based on the results obtained from enzymatic restriction of ITS1, RsaI, and HpaII, and COX1 sequences, all species isolated in the current study were sheep strain (G1). The COX1 gene sequences were registered in GeneBank with accession numbers KY485993 to KY486007. Phylogenetic analysis of COXI sequences showed the highest likelihood with G1 sheep strain using the maximal likelihood method.
Based on the demographic characteristics of the patients, the genotype of the parasite, and host-parasite relationships, the apropriate treatment and tramsmisson pattern are easy to achieve; moreover, proper prevention programs can be performes for rural sectors.