Comparison of Precipitation Reference Method with New Direct Assay of Total Iron Binding Capacity (TIBC)

Total iron binding capacity (TIBC) assay is important investigation of body iron state. The most common method is chemical-precipitation reference method with pre-analysis step. New diagnostic approaches for direct measurement of TIBC have been proposed to streamline and reducing the time of analysis. The aim of study was to compare of accuracy and precision of TIBC direct assay with precipitation method.
Under standard laboratory condition, twenty-five individual's serum samples for each of the low, normal and high TIBC levels were analyzed by both precipitation and direct methods. To investigate of analytical accuracy and precision, the ssayedcommercial serum-control was used.
There were not significant difference between different serum-TIBC levels which analyzed by precipitation and direct methods (P=0.25, P=0.17 and P=0.34 for ow, normal and high levels, respectively); however, there were more differences between serum-TIBC that analyzed by precipitation method. To investigate of within-run -run 9%) for precipitation and direct methods, respectively. However, there were not significant differences in accuracy and precision between two methods (P=0.53).
The results showed that despite of allowable accuracy and no significant differences between precipitation and direct methods of TIBC measurement, because of lower standard deviation and CV%, and also simplicity of direct assay, it is suggested that the direct assay can be replace instead of precipitation method.