Investigating Classes of Integrons in Salmonella infantis Isolated from Clinical Samples and their Antibiotic Resistance Profile

Salmonella is one of the most important food-borne pathogens and zoonotic agents all over the world. Antibiotic resistance is a growing problem in Salmonella isolates and spreading of resistance genes by integrons to susceptible strains is one of the major concerns in the emergence of resistant strains. The aim of the current study was the identification of classes of integrons in the Salmonella infants isolated from clinical samples and their antibiotic resistance profile.
Material & In the cross-sectional study, 101 salmonella isolates were obtained from the stool samples. The antibiotic susceptibility test was determined using the disk diffusion method agreeing with CLSI guideline. Cellular DNA was extracted by AccuPrep Genomic DNA Extraction Kit and MPCR was performed for the identification of the intI, intII and intIII genes.
Findings: Sixty S. infantis isolates were collected using biochemical and microbiological tests. The lowest resistance rate in all isolates was related to Ceftriaxone (45%). The molecular analysis of classes of integrons showed 59 (98.3%), 51 (85%) and 23 (38.3%) isolates caring intI, intII and intIII genes, respectively.
Discussion & The result of this study showed that due to increased level of drug resistance in S. infantis and the presence of class 1 integron in these strains, resistance can be transferred to other food borne pathogens.