A Comparative Investigation of Catalase 2 and Thioredoxin H5 Genes Promoters in Arabidopsis to Determine of Their Functions in Response to Biotic and Abiotic Stresses

In order to investigate of cis-regulatory elementsrole in the promoter region in the expression pattern of genes Catalase 2(CAT2) and Thioredoxin H5(TRX5) in Arabidopsis in response to different biotic and abiotic stresses,the promoter sequences of the genes, cis-regulatory elements in them, data relating to the expression of these genes in the different stresses were received using Phytozome, PlantCARE and the Bio-Array Resource for Plant Biology, respectively. Significant differences in the number of repetitions of each cis-regulatory element among promoters were determined by a variety of statistical methods available at bioinformatics IDEG6 tool. The results showed that eight cis-regulatory elements,including ARE,CAAT-box,ABRE,Unnamed-4,Box-W1,TGA-element,W box and TATA-box had significant and different occurrences between two promoters. It can be suggested that gene TRX5 unlike gene CAT2 is able to respond to biotic stress due to the presence of a greater number of specific regulatory elements in response to pathogen(Box-W1 and W box) and auxin(TGA-element). Also, a part of the expression pattern of these genes in response to abiotic stresses can be justified due to the presence of different number of repetitions of specific regulatory element in response to auxin(TGA-element) on the TRX5 gene promoter and anaerobic regulatory element(ARE) and abscisic acid regulatory element(ABRE) on the CAT2 gene promoter. Based on these results, TRX5 plays a role in response to biotic and abiotic stresses. Identification of these genes with functional response to biotic and abiotic stresses can be considered a huge progress in order to select candidate genes for genetic manipulation to enhance plants stress tolerance.