The study of genetic variation for Lolium perenne using molecular and biochemical markers

Genetic variations were study for 12 accessions of Lolium perenne using molecular and biochemical markers. Soluble proteins were extracted for each population by Laemmli′s method and electrophoresis was done using polyacrilamid gel. The numbers of 12 bands were observed for genotypes, which the numbers of 6 bands were polymorph. The highest number of band belonging to Tyrone with 12 bands and the genotype of Green Gold had the lowest number of band (6 bands). The results of cluster analysis and principal coordinate analysis were fall the genotypes in three groups. Genetic variation for accessions were surveyed using the number of 12 ISSR primers, that the number of 10 primers can be scored. The ISSR primers can be produced the number of 62 bands, which the polymorphism was showed for the number of 47 bands. The average of bands was 6.2 for each primer. The primer of IS9 showed the highest number of band (11 bands) and IS15 showed the lowest number of band (3 bands). A desirable polymorphism between genotype was observed based on ISSR markers, which the primers of IS9, IS10, IS13 and IS16 were determined for genetic variation study in Lolium perenne as desirable primers. Genetic variation based on ISSR marker had a average conformity with the results of protein surveys.