Effect of olive leaf aqueous and hydroalcoholic extract on leishmania tropica Glucantime resistance and sensitive in vitro

Cutaneous leishmaniasis is the most important parasitic disease. As the number of resistant types to glucantime (drug of choice) is increasing, it is needed to investigate the effects of other drugs like herbal medicines on this disease. Several studies have shown the anti Leishmania effects of olive leaf on the Leishmania and also some organisms. The goal of this study was to evaluate the in vitro effects of olive leaf aqueos and hydroalcoholic extract on amastigots of Leishmania tropica glutamimn resistance and sensitive in vitro.
Materials & Methodes: Mouse peritoneal macrophages for the maintenance of Leishmania were used in vitro culture.To determine the effect of the extract on macrophage cytotoxicity MTT assay was performed and CC50 was calculated. In order to investigate the Leishmania tropica amastigots glutamimn resistance with different concentrations of olive leaf aqueous extract 0.31, 0.625, 1.25, 2.5mg/ml and olive leaf hydroalcoholic extract with different concentrations 3.1, 6.25, 12.5, 25 µg/ml were incubated for 24-72 hours. Geimsa stain was used as positive and negative controls, respectively.
we found that olive leaf aqueous extract in 2.5mg/ml and hydroalcoholic extract in 25 µg/ml to 72 hours, are the best concentration and time. It is important to note that olive leaf aqueous and hydroalcoholic extract didn’t have any effect on Leishmania tropica Glucantime resistance in vitro (p≤0.05).
Discussion & the natural products are available and safe and can be used as a drug model without side effects, especially on amastigots of Leishmania tropica glutamimn sensitive.