Interactive effect of treatment with copper sulfate and/or formalin bath with nitrite and ammonia intoxication, on some biochemical parameters of blood serum of Common Carp

This study was performed in order to investigate the effect of treatment with copper sulfate and formalin bath in toxication with nitrite and ammonia, on some biochemical parameters of blood serum of Common Carp. In this study, 400 common carp were used to determine the lethal concentration of nitrite and ammonia and 360 fishes were used to investigate the interactions between drugs and poisoning. To investigate the interaction effects, fish were divided randomly to 12 treatments (each group contained 30 samples) and 3 replicates. The duration of the experiment was 96 hours. At the end of the experiment total protein, glucose, cholesterol, AST, ALT, ALP and HDL were measured in blood serum. According to the results, the LC50 at 96 hours for ammonia and nitrite, were obtained 102.45 and 512.46 mg/l respectively. In the study of treatments, significant differences was observed in glucose, cholesterol, AST, ALT and HDL (p0.05). All the fish of ammonium nitrite copper sulfate treatment in 96 hours were experiencing mortality in the first few hours of the start of the experiment. The results showed that total protein between different treatments and control groups, had not significant difference (p>0.05). The measurement of serum enzymes, most changes were observed in the treatment of ammonia nitrite copper sulfate. Generally, results indicate that the presence of nitrite and ammonia in water and Treatment with formalin and copper sulfate at the same time, it can cause mortality or a significant increase of some enzymes such as AST, ALT and LDH.