The effect of equilibration times on post-thawed buck semen in soy lecithin supplemented extender

The aim of this study was to determine the effects of different pre-freezing equilibration times (2, 4 and 6 h) on quality parameters if post-thawed buck semen using soybean lecithin supplemented extender. In this study, semen samples were collected from four Mahabadi bucks. After collection, semen samples were primarily evaluated and pooled together. Afterward, pooled semen samples were divided into three equal parts and diluted in semen extender containing soybean lecithin. Then, after spending different equilibration times diluted semen samples were frozen. After thawing, sperm motility characteristics, plasma membrane integrity and functionality, abnormality and lipid peroxidation were evaluated. Base on the obtained results showed that six h-equilibration time resulted in higher progressive motility (25.6 ± 1.39 %) compared to other groups (P