Standardization of Molecular Diagnostic of the entC and ent E Staphylococcus aureus Isolated from Human Infections in Zabol

Staphylococcus aureus is one of the most important causes of hospital infections around the world. It has been found in various studies, that 15% - 18% of the Staphylococcus aureus strains isolated from different sources are capable of producing enterotoxin. The aim of this study is identifying Staphylococcus aureus, types C and E, which produce Enterotoxin, from human Infections in Zabol by PCR.
The present study was conducted on 60 urine samples in a 1-year period. Samples were randomly collected from different parts of the city of Zabol and transferred to the university lab in a minimum period of time by maintaining cold conditions. DNA extraction was performed and PCR was done for enterotoxin E and C.
The results of this study showed that isolated S. aureus was resistant to antibiotics including cefazolin (7.3%), doxycycline (12.10%), erythromycin (22.3%), rifampin (9.7%), ciprofloxacin (18.10%), and Co-trimoxazole (14.5%). The results of this study showed that the prevalence of enterotoxin E gene in S. aureus samples was 1.5%.
Regarding the importance of S. aureus secretes, 2 types of toxins with super antigen activity, enterotoxins, of which there are 6 antigenic types (named SE-A, B, C, D, E and G). In case of expression of enterotoxin genes, rapid treatment of the infection seems to be necessary.