Effect of Intensive Exercise Training and Vitamin E Supplementation on the Content of Rat Brain Neurotrophic Factors

Hydrogen Peroxide (H2O2) and Tumor Necrosis Factor alpha (TNF-α) increase Brain-Derived Neurotrophic Factor (BDNF) and Glial Cell Line-Derived Neurotrophic Factor (GDNF) expression. Athletics use anti-oxidant and anti-inflammatory supplementations to reduce H2O2 and TNF-α level. There is insufficient information about vitamin E supplementation on neurotrophic adaptations induced by intensive exercise training. Therefore, the aim of the present study was to investigate the effects of intensive exercise training in combination with vitamin E supplementation on BDNF and GDNF content of rat brain.
In an experimental study, 40 male Wistar rats were randomly assigned to Control (C), Sham (S), Vitamin E (V.TE), Exercise training (ET), Exercise training Vitamin E (ET.TE) groups, with 8 rats in each group. Exercise training on treadmill (27 m/minute) was carried out in combination with vitamin E supplementation (60 mg/kg/day) for six weeks. Statistical significance was determined at P Vitamin E supplementation increased brain vitamin E levels in V.TE and ET.TE groups (P = 0.001). Significant increments in the levels of H2O2 (P = 0.007), TNF-α, (P = 0.001), BDNF (P = 0.001), and GDNF (P = 0.001) in the ET group were shown; however, vitamin E supplementation could not change exercise-induced increases on the above-mentioned factors in the ET.TE group (the p-values were equal to 0.024, 0.001, 0.001, and 0.001 respectively).
Intensive exercise training increases BDNF and GDNF contents in the whole brain of rats, yet vitamin E does not influence neurotrophic adaptations induced by intensive exercise training.