Investigation of the capability of the new storage media in keeping the periodontal ligament cells Viability

Tooth avulsion is defined as the complete displacement of the tooth from its normal position which causes damage to the periodontal ligament structure, cementum, alveolar bone, gingiva, and dental pulp. Since, the milk is storage medium for the short-term storage of avulsed teeth, the purpose of this study was to determine the ability of the new storage media (symphytum Mihan long-life milks) in keeping the Periodontal Ligament (PDL) cells viability.
Following atraumatic extraction of healthy human premolars, the PDL cells were cultured. A certain number of the cells was seeded in each well of a 96-well plate and treated with Mihan long-life milks, Hank’s Balanced Salt Solution (HBSS), composition media (symphytum Mihan long-life milks), and Apada fresh milk for 1, 3, 6, and 24 hours. Dulbecco’s Modified Eagle Medium (DMEM) and dry medium were considered as positive control and negative control media, respectively. Cell viability was determined by using the MTT assay. Moreover One Way-ANOVA and post Hoc (LSD) test were used for comparing the study groups.
After 24 hours, the cell's viability in composition media was significantly better than Apada fresh milk medium (P=0. 03). Moreover, the results of One-way ANOVA showed that the PDL cell's viability in composition media was higher than the HBSS medium after 24 hours. At this time point, the mean of viable cells in composition media, Mihan long-life milk medium, and HBSS was 135%, 64%, and 35%, respectively.
According to the study results, composition media (symphytum Mihan long-life milks) are considered as desirable storage media which are comparable to HBSS. This media are not only able to keep more cells viability after 24 hours time period compared to the expensive commercial solutions, but they can also be easily accessed and used.