Isolation of tetD, tetC, tetB, tetA‎ genes from Acinetobacter bummani samples isolated from hospital samples by multiplex PCR method

Acinetobacter is a gram negative bacterium seen as cocci or cocobacilli. This opportunistic ýbacterium plays a very important role in hospital infections. Tetracycline is a broad-spectrum ýantibiotic that inhibits the growth of many gram-positive and negative bacteria. Today, antibiotic ýresistance is not responding to the frequency of pathogenic bacteria and the risk of antibiotic ýresistant strains. The aim of this study was to determine the prevalence of antibiotic resistance and ýthe distribution of tetracycline (tetD, tetC, tetB, tetA) genes in Acinetobacter bummani and ýantibiotic resistance Disc diffusion.ý
Subjects & Acinetobacter bummani were isolated from clinical specimens and then identified by using bacteriological and biochemical tests. An antibiotic test was performed using CLSI standard method. By the PCR method, was investigated the presence of tetracycline resistance genes.
The highest resistance to acinetobacter isolates was resistance to ciprofloxacin. Out of 60 isolates of acinetobacter, the prevalence of tetA, tetB, tetC and tetD genes was 91.6, 91.6, 15, and 0%, respectively. This study concludes that resistance genes are present whether or not resistance genes are susceptible to many antibiotic groups.
The main mechanisms for resistance to tetracycline are through the acquisition of the tet gene with the activity of the phallus pumps, ribosomal protection and enzymatic inactivation. An antibiotic resistance can be prevented by identifying antibiotic resistance patterns and using appropriate antibiotics when there is a need for treatment and preventing the release of resistant strains in the community among human populations.