Nucleotide sequence analysis of phylogenetic and evolutionary status of BLG gene

Aim and This study was performed to determine the evolutionary and molecular trend of BLG gene nucleotide sequence in mammals, phylogenetic analysis and the trend of natural selection.
The nucleotide sequence of the BLG gene in studied species were obtained and balanced from genome database (NCBI). Substitution and replacement percent of nucleotides were obtained using maximum likelihood, and phylogenetic tree drew using Neighbor-Joining method.
The results showed that the transitional substitution was more than transversional substitution. The numerical value of dN/dS was 0.70, indicating negative selection during evolution of this gene. The results of phylogenetic tree for this gene showed different evolutionary routes in different species including five categories such as (cow and buffalo), (sheep and goat), horse and wild boar, in which cow, buffalo and sheep had the most relationship.
Mutations and natural selection have been resulted in the development of new varieties, new proteins and also stabilizing their performance during the evolution and progress toward purification of their performance.
According to the results of this study, protected areas constitute a small part of LGB gene sequence which reflects the polymorphism of this gene as well as being susceptible to variations and mutations.