Investigating the possibility of using Lactobacillus plantarum and Lactobacillus kunkeei isolated from honey in the preparation of probiotic pomegranate juice

Bee plays an important role as a useful insect in nature due to pollination. The gastrointestinal tract of the bee has coexist microorganisms. In this study, probiotics Lactobacillus Kankei and Lactobacillus plantarum isolated from honey were prepared and after diagnostic tests including acid resistance, bile salts resistance, gastric juice resistance (pepsin, trypsin), lack of hemolytic activity, hydrolysis of L–arginine were added at the rate of 107 and 108 CFU/ml to pomegranate juice individually and in combination and pomegranate juice tests including acidity, pH, evaluation of soluble solids content (Brix) and evaluation of viability of probiotic bacterial in days zero, day seven, day fourteen and twenty one days was conducted on pomegranate juice. According to the results both microorganisms exhibited probiotic properties.The results of pomegranate juice tests showed that with increasing the storage time and probiotic population, the rate of acidity increased and the rate of pH and brix significantly (p