Evaluation the Glucose Isomease Enzyme Effect on Glucose Solution and Hydrolysis of White Sugar by Invertas Enzyme for High Fructose Syrup Production

Today demand for the production of various high fructose syrup production was increased for application in food and pharmaceutical industries. So the aim of this study was increased the amount of fructose and sugar solution. At first the ratio of enzyme glucose isomerase to the glucose substrate (0.05, 0.1 and 0.2) was studied. Based on results the lowest amount of conversion of glucose to fructose (15%) was in 0.1 proportion and the highest amount of conversion (25%) was in 0.2 proportion. Although no significant diffrence was observed in 0.1 and 0.2 ratio (p≤0.05). Also the results indicated the convesion of glucose to fructose by glucose isomerase during 1, 2, 4, 6, 8, 10 and 12hr were respectively 1.09, 3.17, 5.44, 6.29, 7.20, 7.81 and 8.03%. The results of white sugar hydrolysis by invertas was acceptable and the content of sucrose was in lowest amount and the glucose and fructose were in highest level at the end of hydrolysis. It should be noted that the amount of glucose over than fructose by invertas enzyme after 4hr hydrolysis. Also the results showed the ratio of sucrose to raw sugar in 1, 2, 3 and 4 hr were respectievly 52.62, 52.80, 61 and 69 and the proportion glucose to fructos were 1.62, 1.78, 1.53 and 1.58 %.