Evaluation of the Effects of the Concentration of Ozonated Water on the Inhibition of Candida albicans Colonization and Plaque Formation on Acrylic Dental Plates

The formation of dental plaques is one of the underlying causes of denture-related stomatitis. This study was conducted to evaluate the effect of the concentration of ozonated water on the inhibition of the formation of Candida albicans plaque on the acrylic resin pieces.
In this study, 45 resin acrylic plates were contaminated by Candida albicans suspension, which were randomly divided into nine groups. They were treated with 0.2, 0.5, 1, and 2 µg/ml of ozonated water, 25 and 12.5 µg/ml of ozonized oil, 100,000 units of nystatin (positive control), distilled water, and olive oil (negative control). Thereafter, the solution obtained from rinsing the pieces was cultured in Sabouraud Dextrose Agar and the mean number of the colonies was Mann-Whitney U and kruskal-wallis tests were for statistical analysis.
The mean number of the colonies obtained at the concentrations of 0.2, 0.5, 1, and 2 μg/ml of ozonated water was 24, 24.6, 23.6, and 14.4 colonies, respectively. Additionally, the mean number of the colonies obtained at the concentrations of 12.5 and 25 μg/ml of ozonized oil was 0 and 2 colonies, respectively. There was a significant difference between the solutions cultured in distilled water (146.6) and olive oil (98.8) in terms of the number of colonies (P Appropriate concentrations of ozonated water had an antifungal effect on Candida albicans. Therefore, further clinical studies are recommended to standardize and elaborate the guidelines for using ozone therapy.