Study of the effect of wheat germ oilon carbon tetrachloride induced hepatic damage in adult male mice

Carbon tetrachloride, an organic solvent and chemical intermediate used in the industry, can cause numerous side effects including hepatotoxicity. This study was aimed to evaluate the possible hepatoprotective potential of wheat germ oil against carbon tetrachloride-induced hepatic disorders in male mice.
48 male mice were randomly divided into 6 groups of 8 and treated for 4 weeks including control, carbon tetrachloride (1 ml/kg body weight intraperitoneally, 2 times a week),carbon tetrachloride (1 ml/kg body weight intraperitoneally) wheat germ oil (250 mg/kg body weight per day orally), carbon tetrachloride (1 ml/kg) wheat germ oil (500 mg/kg/day orally), wheat germ oil (250 mg/kg body weight per day orally) and wheat germ oil (500 mg/kg body weight per day orally) groups. At the end of the treatment, animal blood was collected to measure liver enzymes including AST, ALT and ALP. Hepatic tissue samples were harvested, fixed in 10% formalin, stained with hematoxylin and eosin and analyzed using optical microscopy. The data were analyzed by one-way ANOVA and Tukey's post hoc test.
In mice receiving carbon tetrachloride combined with wheat germ oil (250 and 500 mg/kg), liver enzymes (ALP, ALT and AST) significantly (p These findings suggest that wheat germ oil can exert hepatoprotection in carbon tetrachloride evoked hepatotoxicities in mice.