Differentiation potential of human CD133 and CD34 positive hematopoietic stem cells into motor neuron- like cells; an in vitro study

We were studied on differentiation potential of CD133 and CD34 hematopoietic stem cells into motor neuron like cells.
CD133 and CD34 HSCs were isolated from human UCB using MACS system. After cell characterization using flow cytometry, the cells were treated with a combination of Retinoic acid, Sonic hedgehog, Brain derived neurotrophic factor, and B27 through a 2- step procedure for two weeks. The expression of MN-specific markers was examined using immunocytochemistry and flow cytometery.
Flow cytometery analysis revealed 98% and 95.7% CD133 and CD34 cells, respectively. By the end of the two-week differentiation protocol, CD133 and CD34 cells acquired unipolar MNL morphology with thin and long neurites. The expression of Isl-1, AChE, NF-H and Nestin was detected in 66.4%, 58.3%, 80.6%and 84.9% of CD133 cells and 63.2%, 52.3%, 78.6% and 80.1% of CD34 cells. T
Human UCB - CD133 and CD34ᴠ are remarkably potent cell candidates to trans- differentiate into motor neuron-like cells, in vitro.