Investigating the Effects of All-trans Retinoic Acid on Histopathology of Pancreas of streptozotocin -Induced Diabetes in C57BL/6 mice

Backgrounds & Type 1 diabetes is an auto-immune disease and caused by insufficient insulin production by the body. All-trans Retinoic Acid (ATRA) is an antioxidant, anti-cancer and anti-inflammatory agent. The objective of the present study was to investigate the effects of ATRA on histopathology of pancreas in diabetic mice.
Material & Diabetes was induced by multiple low-dose of streptozotocin injection (40 mg/kg/day for 5 consecutive days) in male C57BL/6 mice. After induction of diabetes, mice were treated with ATRA (20 mg/kg/day i.p.) for 21 days. On the last day, pancreases were isolated and stained with hematoxylin &eosin (H&E) and Gomeri aldehyde fuchsin (GAF) for histological analyses (the number of islets and β cells, diameter of islets) of pancreas.
ATRA treatment in streptozotocin-induced diabetic mice was increased the mean diameter of islets and the number of islets and beta cells compared to the diabetic group. (p The administration of ATRA improved pancreas tissue during destruction of the pancreatic beta-cells in STZ-induced type 1 diabetes in mice.