Molecular epidemiology of MBL-Producing Acinetobacter baumannii in the west of Iran

Carbapenem-resistant A. baumannii has been isolated highly in recent years. Metallo-b-lactamases (MBLs) have been detected and reported from A. baumannii producing carbapenemases in recent times. The aim of this study was to determine both phenotypic and Genotyping of MBL producing A. baumannii isolates.
A total of 110 Acinetobacter spp. were cultured from clinical specimens of hospitals of Kermanshah in western. To screen for the MBLs, E-test strips (AB Biodisk, Solna, Sweden) were used according as per the manufacturer’s instructions. DNA genomic of A. baumannii isolates were SmaI-digested and analyzed by CHEF Mapper PFGE
89 (80.9%) of isolates producing MBL by E-test MBL. Of 89 MBL producing A. baumannii tested were recovered from ICU 68 (76.4%), emergency wards 16 (17.9%), and pediatric wards 5 (5.6%). Among 47 MBL producing A.baumannii that selected for Pulsed-field gel electrophoresis analysis, we obtained 7 pulsotypes that including 4 common types and had 3 single types.
MBL producing A.baumannii has severely limited therapeutic options. The high MBL producing isolates found in this study may be associated with the high frequency at which these antimicrobial drugs were used for both prophylactic and therapeutic treatment of hospitalized. Our study showed that most of the isolates of A. baumannii were obtained from ICU and most members of clone A are collected from this ward. Present of CloneA in ICU ward is warning.