Histopathology of Propolis Ethanolic Extract (EEP) effects on inflammatory reactions in Experimental Cutaneous Candidiasis in goats

During the history, researchers studied the healing effects of traditional medicine against many infectious diseases. In order to explore therapeutic role of Propolis Ethanolic Extract (EEP), an experimental cutaneous candidiasis was induced in goat model as InVivo study.

Four young healthy goats without any skin fungal infection were selected from different herds. The skin on both sides of the lateral thoracic and flank areas of the studied animals were shaved. The Candida albicans PTCC 5027 suspension were inoculated on each site. On day 8th, 64 large abscesses and pimples were appeared on all inoculated sites. All treatments begun on 8th day. Wideness of Cutaneous Candidiasis lesions on all sites of all goats scaled by a ruler during this study in centimeter and monitored planimetrically and analyzed statistically. Biopsy were collected on particular days under local anesthesia after intradermal inoculation date. Sections were stained with hematoxylin and eosin (H & E) and special stain of G.M.S. for fungal infection.

Comparison of the values for different treatments revealed a significant reduction in abscess size and inflammatory signs in EEP treated group comparing to Nystatin treated , Glycerin treated and untreated groups (P≤0/05). Histological findings showed anti-inflammatory results, with better cutaneous repair, sebaceous, sweat gland, and hair follicle regeneration in EEP group, better than other treatment groups (P≤0.05).

EEP has a considerable anti-inflammatory and antifungal effects even more better than Nystatin ,without its side effects (scar formation, shedding of infected crust, "treatment dependent hypersensitivity").