Effect of Low Level Laser Irradiation on the Function of Glycated Catalase

The aim of this work is to evaluate the effect of low level laser irradiation (LLLI), by lasers with different wavelengths, on glycated catalase enzyme in vitro experimentally. This is done by measuring the activity and structure properties of glycated catalase enzyme. The structure properties were evaluated with circular dichroism (CD) and fluoroscopy methods. Three continuous wave (CW) lasers in visible spectrum (λ= 450, 530, 638 nm) and a 100-ns pulsed laser in infrared spectrum (λ= 905 nm) were chosen for comparison. For the infrared laser, same effects have been investigated for different energy doses. The effect of photon energy (hυ) at different wavelengths was measured on activity, CD, and fluoroscopy properties of catalase, and compared with the control group [samples without irradiation]. The energy intensity of laser should not exceed 0.1 J/cm2. Experiments were performed on glycated catalase between 2 to 16 weeks after glycation of catalase. The LLLI effect has also been investigated on the samples, by comparing the catalase activity, CD and fluoroscopy for different wavelengths.
Our results indicate, the decrease in catalase activity as a function of glycation time (weeks) for all samples, and a slight increase on its activity by different laser wavelengths irradiation for any fixed period of glycation time. Finally, as the laser’s photon energy (hυ) increases, the catalase activity also increases. More specifically, the blue laser (λ= 450nm) has the most and the red laser (λ = 638nm), has the least effect, and the green laser (λ = 530nm) has the medium effect on catalase activity. Furthermore, pulsed laser had an additional effect by increasing energy dosage. As we expected in all experiments, the increase in the catalase activity was coincident with the decrease in catalase fluoroscopy and CD parameters.