Cytotoxicity and apoptotic effect of oxaliplatin on colon cancer cell line (HT29) and analysis of caspase 3 and caspase 9 gene expression using Real Time PCR method

Aim: The aim of this study is investigation of cell toxicity of oxaliplatin on colon cancer cell line and analysis of caspase 3 and 9 apoptotic genes.
Material and In this experimental study, cytotoxicity of oxaliplatin on colon cancer cell line (HT29) was evaluated using MTT method in different concentrations including 100, 50, 25, 12.5, 6.25 and 3.125 µg/mL and the IC50 value was determined. After treatment of HT29 cells with IC50 value, the cells RNA was extracted and converted to cDNA. Subsequently, the expression level of caspase 3 and caspase 9 apoptotic genes comparing to house-keeping gene (β-actin) was measured using Real Time PCR.
Treatment of HT29 cells with various concentrations of oxaliplatin including show that oxaliplatin has the highest cytotoxic effect in 100 µg/mL, which is statistically significant (P According to cell toxicity and apoptosis induction in HT29 cells by oxaliplatin, it can be concluded that this drug is an appropriate choice for treating of colon cancer.