Genetic evaluation of different population of Cumin (Cuminum cyminum L.) using DNA molecular markers

in this study tries to kinship and genetic distance between populations is assessed cumin. A total of five population of cumin were collected and then were analyzed using 10 RAPD primers and 10 ISSR primers. To analysis of data will be used NTsys pc2.1, GenAlex6.501 and Popgene32 software. The RAPD primers gave 182 Amplified bands that TIBMBC03 primer had the highest bands (24), marker index (12.15) and diversity index (0.5) and The ISSR primers gave 135 Amplified bands that UBC809 primer had the highest bands (18), marker index (7.56) and diversity index (0.47). In RAPD marker, the highest Nei diversity (0.32) and Shanoon diversity (0.333) was belong to cumin of Sabzvar and also in ISSR marker the highest Nei diversity (0.18) and Shanoon diversity (0.27) was belong to cumin of Sabzvar. According to RAPD marker, the lowest value of similarity (0.114) between Sabzvar and Kashan Cumin and also in ISSR primer, the lowest value of similarity was observed (0.085) between Sabzvar and Kashan Cumin. Moreover, according to both ISSR and RAPD analysis, the lowest genetic similarity was 0.117 between Sabzvar and Kashan Cumin. The results of this study showed that Sabzevar cumin is recommended as a center of plant diversity on the basis of studied populations and the farthest population to earliest possible crosses and create a variety of genetic diversity were cumin of Sabzevar and Kashan.