Extensive outbreak of the type A of Beet Necrotic Yellow Vein Virus in Iran

The present study aimed to evaluate different Beet necrotic yellow vein virus (BNYVV) genotypes in sugar beet research stations in Iran including Toroq, Zarqan, Miandoab and Ekbatan. Some samples were also collected from sugar factories’ field in Torbat-e Jam, Nahavand, Kamalabad, Naqadeh and Qazvin and also sugar beet fields in Arak. A total of 58 infected sugar beet root samples were collected. In addition, 30 soil samples were collected from sugar beet fields in Moghan, Toroq, Zarqan and Kamalabad, and a susceptible cultivar was planted in them to determine the causal agent type. To detect type A or B of the virus, TGB region of the virus genome was multiplied using multiplex-PCR. Moreover, partial multiplication of RNA5 using RT-PCR and specific primers was performed to detect type P or J. Only 324 bp fragment was multiplied using multiplex-PCR and specific primers designed for type A or B. Therefore, among 88 samples, only type A was detected. No BNYVV isolates containing RNA5 was observed in any of the samples.