The Effect of Tricine Compound Extracted from Allium atroviolaceum Boiss. On growth and apoptosis in PC3 cell line

Tricine, as a flavonoid compound in many food sources, has anti-inflammatory and anti-proliferative effects in some cancer cell lines. Considering the importance of using natural anti-cancer drugs in therapy-resistant cancers such as prostate cancer, the aim of this study is to investigate the effect of tricine on cell growth and proliferation and induction of apoptosis in PC3 human prostate cancer cell line.
In this experimental study, the PC3 cell line was prepared and cultured from the Pasteur Institute of Iran. Extraction and purification of tricine were done by column chromatography and recrystallization of Allium atroviolaceum extract. The apoptotic effect of tricine at concentrations of 60, 80, 100, 120 and 140 μM was evaluated by MTT method. The apoptotic effect was evaluated in the cell group treated with IC50 concentration of tricine and untreated cells (control group) using Annexin-V kit and flow cytometry.
FINDINGS: The viability of cells at different tricine concentrations were 85.66±1.52, 76±3.60, 66.33±4.16, 44±3.60, and 36.66±3.21, respectively (p The results of this study showed that tricine resulted in cell death in the PC3 cell line, but the cell death mechanism was not apoptotic.