Leukogram changing of Holstein calves response to acute inflammation due to experimental coisepticemia

Colisepticemia is an acute fatal disease that occurs primarily in calves less than 2 week’s age and in calves with failure of passive transfer, and cause heavy economic losses to husbandry industries. Primary signs of septicemia is nonspecific and cannot be differentiated from non-infectious diseases or diseases with local infection such as diarrhea. Leukogram is a key laboratory tool to evaluate neonates suspected to septicemia in which supplies valuable diagnostic information for clinician next to physical examination. In this study, colisepticemia was induced with Escherichia coli strain of O111:H8 in 10-day bull Holstein calves weighting 50±50 Kg. so, suspension of bacteria in normal saline, containing 1.5× 109 CFU, was given intravenously through the jugular vein. Leukogram changing was examined from before 24 hours of bacterial injection till 24 hours after that. Leukopenia, neutropenia, lymphopenia, eosinophilia, thrombocytopenia and increase of PCV, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and hemoglobin, and decrease in red blood cell count was observed significantly associated with septicemia beginning (P 0.05). Total protein: fibrinogen ratio altered from >15 at 0h to