Evaluation of probiotic properties of human milk lactobacilli by PCR assay
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Human breast milk contains high amounts of necessary nutrients such as carbohydrates, essential fatty acids, proteins, vitamins and minerals for infants. It is also considered as a continuous source of commensal and potentially probiotic bacteria for infant gut which are leading development and composition of the neonatal gut microbiota for treatment and prevention of intestinal infections. The aim of this study was to evaluate prevalence of lactobacilli existing in breast milk that isolated from healthy women and their potential probiotic properties. In this study, 122 lactobacilli were collected from 100 human breast milk samples. Isolates were identified based on morphology and their results to catalase test and gram stain. The ability of tolerance acidic pH and resistance to bile salts were applied to confirm probiotic potential. Molecular identification was carried out with 16SrDNA and Polymerase Chain Reaction. As 100 samples, 122 Lactobacilli were identified. The most dominant isolates were belonged to the species of casei and rest of them to the species of fermentumplantarum and gassery respectively. 19 isolates were verified with probiotic properties. Molecular studies with 16SrDNA, guaranteed our outcomes. Breast milk is a rich source of lactobacilli with probiotic properties. Since these isolates are safe with human origin, they could be utilized as an additive in food industry and pharmaceutical fields. Administration of probiotic lactobacilli in formula milk, may be useful in order to prevention of infant’s infections.
Language:
Persian
Published:
Journal of Applied Microbiology in food industry, Volume:4 Issue: 1, 2018
Pages:
25 to 33
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