Evaluation of antibacterial properties of alcoholic extract of pine on bacteria isolated from urine of patients with urinary tract infection - genital

Urinary tract infection is one of the most common infections and the second cause of infection in human body. The main cause of this infection is gram negative bacteria such as E coli. The base of treatment for urinary tract infection is administrating an appropriate antibiotic. Today, antibiotic resistance is considered as an important challenge in treating infections. High and unnecessary use of antibiotics especially in urinary infections resulted in resistance to some antibioticsThis study aimed to evaluate the antibacterial effect of alcoholic extract of Escherichia coli took over the pine leaves.
In this experimental study the Alcoholic extract was extracted using. its antimicrobial activity agar disk diffusion method for determination of bacterial sensitivity and dilution method for determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) is used separately.
Also the results of MIC and MBC tests indicated that the inhibition of E.coli and Staphilococci aureus bacterial growth by Alcoholic extract of pine had a direct relationship with the amount of available alcoholic extract of pine. with increasing of Alcoholic extract of pine in each dilution, reduced the number of cultured bacterial colonies and no bacterial growth was observed in the dilution equivalent to MBC of Alcoholic extract of pine.
The results suggest that Alcoholic extract of pine can be used as a source of cheap and accessible replacing chemical drugs to treat some bacterial infections.