Investigation of Antioxidant Effect of Unsaponifiable Matter from Cannabis Sativa L Oil on Stabilizing of Soybean Oil

With the concern of adverse effects of lipid oxidation on food deterioration and human health, the antioxidant activity of unsaponifiable matters (USM) of hemp seed oil on soybean oil were evaluated by comparing peroxide value, carbonyl value, polar compounds and oxidative stability in thermal condition.Unsaponifiable matters of hemp seed oil was obtained 1.31% which tocopherol, strole and phenolic compounds were 178.73 mg/100g, 2779.36 mg/kg and 6832.88 mg GA/100g, respectively.The investigation results of antioxidants activity of USM using DPPH radical scavenging method, βcaroten:linoleic acid bleaching assay and ferric reduction antioxidant power showed that by increasing in USM, antioxidant activity were increased and USM in 200 ppm was similar to 100 ppm of TBHQ. In thermal conditions, control samples showed higher oxidation indexes and antioxidant activity of USM was higher than TBHQ. These results of this study suggest that USM of hemp seed oil may be used as potential source of natural antioxidant in the application of food industry to prevent lipid oxidation.