Optimization of callus induction in balck seed (Nigella sativa L)

Black seed is one of the most important medicinal plant in the family Alaleh. This plant content of different medicinal compounds which is used to produce a variety of medications for the treatment of diseases. According to the value of the medications of this plant, limited studies have been done on tissue culture. In the present study, the rate of callus induction was provided of leaf, meristem and shoot of black seed on medium culture MS containing of growth regulator BAP and 2,4-D, respectively in three levels of 1, 2 and 4 mg/l of 2,4-D hormone and 0.1, 0.2 and 1 mg/l of BAP hormone was investigated. Each of growth regulator was studied as a factorial experiment in a completely randomized design with three replications on MS medium. After each subculture period traits fresh weight and dry weight were measured. The result of analysis of variance showed that interaction of growth regulators and explant for the studied traits first and second subculture were significant. The result of comparison of mean showed that the best treatment in first and second subculture BAP hormone belongs to leaf explant in concentration 1 mg/1 were fresh weight 0.266 and 0.224 g respectively. In growth regulator 2.4-D was observed the best treatment belongs to leaf explant in concentration 4 mg/l were fresh weight 0.233 and 0.766 g respectively.