Effects of Biopriming with Trichoderma Fungi on Germination and Some Vegetative characteristics of Soybean and Canola

In order to investigate the effect of biopriming with selected species of Trichoderma on the morphological and germination characteristics of Canola (Brassica napus) seedlings, and soybean (Glycine max) cultivar Williams, an experiment was conducted in a randomized complete block design with three replications. The effect of gamma-mutation on Trichoderma genome and its effectiveness was investigated. Experiment with three levels of inoculation: control (non-inoculation), Inoculation with formulations from spores of wild type Trichoderma and inoculation with mutant isolates of the same species, at the Nuclear Agriculture Research Institute was carried out. Evaluation of germination indices (Germination percentage, stem length and root and seedlings and their fresh and dry weight and vigor index) based on ISTA standard implemented. Soybean bioprimed seeds with Trichoderma in most of measured indicators had significant difference with other treatments and growth components, especially in initial development and early growth stages were improved. In Canola seeds, all germination indices were decreased by biopriming with Trichoderma and in the most of the indices, there were no significant differences between wild type and mutant Trichoderma treatments. Overall, the results of this study showed, using seed biopriming with Trichoderma had different effects on plant growth parameters, and use of this technique needs case study for each plant genome.