Separation of Proline Amino Acid by Aqueous Two Phase Sysytem

Aqueous two- phase system is a well-known technique for separation of bio-molecules such as proteins and enzymes. Proline as an Amino acid has a lot of applications in different industries. In this research, proline partition coefficient in aqueous two-phase system of polymer- salt has been investigated. The system consists of polyethylene glycol as a polymer with molecular weights of 1000, 2000, 3350, 6000, 8000 and a biodegradable salt that is suitable for the environment, D-potassium tartrate. Phase diagram (binodal curve) and tie lines were experimentally determined with different percentage of weight composition of polymer, salt and water from initial feed. Analysis of surface of two-phase area in binodal curve and tie line length were done. The best polymer and salt stock solution were suggested for phase diagram, and in that case increasing PEG molecular weight increases surface of two-phase area. Effect of PEG molecular weight, tie line length, polymer and salt concentration on proline partition coefficient has been investigated.