Effects of various concentrations of hormonal treatments on In vitro culture of red seedless grape

Red Seedless grape is one of the most important and qualitative Iranian grape varieties which has been welcomed by farmers recently. Given the importance of the mass propagation of this plant, producing healthy seedlings, and particularly free of grapevine crown gall disease or cancer, in the present study the effects of various hormonal concentrations treatments were studied on proliferation and rooting of red seedless grape, with the aim of giving a suitable instruction for in vitro culture of the plant. For this purpose, the MS medium containing various concentrations of benzyl amino purine (BAP) (0.25, 0.5, 1, and 2 mg L-1) with 0.2 mg L-1 indole acetic acid (IAA) were used for proliferation and various concentrations of indole butyric acid (IBA) (0, 0.8, and 1.6 mg L-1) were used for rooting of the samples in a completely randomized design. The results showed that the studied hormones had the significant effects on the characteristics such as node number, nodal length, shoots number, length, diameter, fresh and dry weight, leaf number, leaf area, chlorophyll index, root number, length, fresh and dry weight. There was also different between concentrations of the used hormones. According to the results the highest proliferation and rooting was achieved at 0.25 mg L-1 of BAP and 0.8 mg L-1 of IBA, respectively. According to the results, these hormonal treatments can be useful for proliferation and rooting of red seedless grape.