Effect of Phenylhydrazine-induced hemolytic anemia on morphological changes of renal cortex in adult mice: Protective effect of crocin
Message:
Abstract:
Background
Hemolytic anemia and resultant hypoxia can cause a dysfunction in the body functions. Iron delivered from hemolysis of erythrocytes can also stimulate oxidative stress.
Objectives
In this study, we aimed to investigate the protective effect of crocin on renal quality in animals that were exposed against hemolytic anemia induced by phenilhydrazine.
Methods
Forty-nine male and adult mice (20 – 25 g) were grouped within 7 groups. First group was control and treated with normal saline, and test groups were nominated as 2, 3, and 4 and were treated with phenyl hydrazine 2, 4, and 6 mg/100g/48 h (i.p.). Animals in groups 5, 6, and 7 were treated with crocin (200 mg/k/day, i.p.) in addition of 2, 4, and 6 mg/100g/48 h phenyl hydrazine. After 35 days, the blood samples were collected directly from the heart and after centrifugation (5 min and 3000 rpm), the serum samples were subjected to evaluate the Malondialehyde (MAD) level and total antioxidant capacity (TAC), and renal were fixed in 10% formal saline for Paraffin sections. After tissue processing sample staining with H&E method.
Results
This study showed that hemolytic anemia effects in proximal tubule of renal cortex, elso showed that the phenylhydrazine administration resulted in a dose-dependent declining of TAC and the crocin administration slightly was able to protect from TAC reduction. Moreover, PHN increased MDA level in serum in a dose-dependent manner, while co-administration of crocin with PHN significantly (P
Conclusions
These results indicate a protective effect of Crocin against the anemia-related hypoxia on renal parameters. The protective capacity of Crocin might relate to its known antioxidant power.
Article Type:
Research/Original Article
Language:
Persian
Published:
Journal of Veterinary Research, Volume:73 Issue: 1, 2018
Pages:
63 - 70
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