Exopolysaccharide Production by Bacillus Pasteuri and the Study of its Antibacterial and Antioxidant Properties

Aim and Background: The exopolysaccharides are biopolymers which are secreted to the surrounding environment by microorganisms. The aim of this research is to determine situations for higher production of exopolysaccharide by bacillus pasteuri bacterium and to study its antibacterial and antioxidant properties.
Material and methods: In this study, experimentation has been performed based on taguchi method and by the use of Qulitek-4 software to determine the situation of exopoysaccharide. Then, different degrees of thickness were obtained from the produced exopolysaccharide and were applied to staphylococcus aures and E. coli bacteria, in order to study the effects of its antibacterial properties. In order to study the antioxidant activity, 4 different degrees of exopolysaccharide were prepared and were measured by three methods the scavenging activities of DPPH, hydroxyl radicals and superoxide anion radical.

The best situation for the highest level of production of exopolysaccharide was after 48 hours from the time of bacterium cultivation with the salt result of 0/05 milligrams on milliliter and the sucrose of 1 milligram on milliliter. The results coming from antibacterial activity showed that the highest halo of non-growth was related to the 400 µg/ml thickness. The results of antioxidant activity in all the three methods used in this study showed that in the thickness of 1000 µg/ml from exopolysaccharide exists the highest controlling activity.

By identifying several key factors we can produce the highest level of exopolysaccharide by the bacillus pasteuri bacterium and we can use it in antibacterial and antioxidant activit.