Identification of extermophilies producing l- glutaminase and l-asparaginase from Larijanhotspring

The most frequently used sources of more stable enzymes are thermophilic bacteria. In recent years microbial L-asparaginase has been widely used as therapeutic agent in the treatment of human cancers. Microbial L-glutaminase is currently used in the treatment of acute lymphoblastic leukemia (ALL) and human immunodeficiency virus (HIV) because of its potential applications in medicine . The aim of present study was to screen new potent thermophilic bacterial isolates producing L-asparaginase and L-glutaminase. Larijan hotspring was chosen as the location for isolation of the thermophilic bacteria. Culture and morphological and biochemical tests, were performed. All the bacterial isolates were screened in ortherto L-asparaginase and L-glutaminase enzyme activity using Rapid Plate assay method. M9 modified media incorporated L-asparaginase or L-glutaminase and phenol red as indicator were used. Thermophilic strains were later identified by 16SrRNA gene ribotyping followed by PCR . A total of six isolates recovered from Larijan hotspring were found to belong to Bacillus licheniformis. The L-asparaginase activity was observed in LT-1, LT-2, LT-3, LT-4, LT-5 strains.The L-glutaminase produced by Bacillus licheniformis LT-1, LT-3, LT-5 and LT-6 strains. Three thermophilic LT-1, LT-3, LT-5 strains with L-glutaminase and L-asparaginase activity have been successfully isolated. Larijan hot spring is one of the potential sources of useful microbial products that can be used as a source of useful different biological products like enzymes.