The effect of lecithin animals, plants on properties of bovine serum albumin cryopreservation and bull semen fertility
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Background and objectives
The storing and cryopreservation of livestock semen for long time cause using possible benefits of the best genetic reservoirs, but during cryopreservation, damages from peroxidation and cold shock lead to a lowered semen fertility. So, using compounds in semen extender effective on fertility is pivotal. In this study, three experiments were conducted to investigate effect of different soy lecithin instead of egg yolk in Tris extender and also adding bovine serum albumin on fertility traits of bovine semen stored under three thermal conditions; room temperature (24°C), chilling temperature (5°C), and frozen in liquid nitrogen.
Materials and methods
semen samples were collected from four Holstein bulls (about 3 years old) using artificial vagina and pooled after initial quality evaluation. Semen samples then were extended by four different extenders containing egg yolk (20%) and three levels of soy lecithin (0.5, 1 and 1.5%). Before filling 0.5ml straws with extended semen, 10mg bovine serum albumin was added to the half of straws. Straws were stored at three thermal conditions; under room temperature and evaluation at hours 3, 6 and 9, under chilling temperature and evaluation at hours 3, 24 and 48, and under freezing condition and evaluation of thawed straws at days 5, 10 and 15 post-freezing. Sperm concentrations, viability and motility were evaluated microscopically. Data were analyzed as a 2×4×3 factorial experiment (including extender, bovine serum albumin and time effects) with a completely randomized design. Means comparison was by the least significant difference test.
Results
Results from the experiment under room temperature showed that the effects of extender and time on sperm viability and motility were significant (p<0.01) but adding albumin had no significant effect on that. The highest sperm viability and motility percentages were 99.88 and 99.75 in egg yolk group at h 3, respectively. Regarding to the results from chilling condition, the effects of extender and time on sperm viability and motility were significant (p<0.01) but adding albumin showed no significant effect. The highest sperm viability and motility percentages were 98.34 and 97.67 in egg yolk group at h 3, respectively. The effects of extender and time on sperm viability and the effect of extender, albumin and time on sperm motility after thawing were significant (p<0.05). The highest sperm viability and motility percentages were 68.18 and 65.62 in egg yolk group at day 5 post-freezing, respectively.
Conclusion
Generally, results of the study showed that in Tris semen extender, using egg yolk than soy lecithin has priority for storing bull semen in liquid and frozen conditions. Furthermore, adding bovine serum albumin into extender is not effective on bovine sperm viability and motilityKeywords:
Language:
Persian
Published:
Journal of Ruminant Research, Volume:6 Issue: 1, 2018
Pages:
147 to 154
magiran.com/p1884112
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!