Relationship of 536C/T Tissue Factor Pathway Inhibitor Gene and Alu_I/D Tissue-type Plasminogen Activator Gene Polymorphisms with Recurrent Pregnancy Loss in Iranian Azeri Women

Aims: Tissue factor pathway inhibitor (TFPI) and tissue plasminogen activator (tPA) deficiency would cause an increase in the viscosity of fibrinogen and low digestion of blood clot, respectively, which are among the dangerous factors responsible for the increase in thrombosis. Although there are reports, indicating the relationship of vein thrombosis and recurrent pregnancy loss (RPL), the effect of polymorphisms connected with thrombosis on the risk of increasing RPL has remained unclear. Thus, the aim of this study was to evaluate the relationship of 536C/T tissue factor pathway inhibitor gene and Alu_I/D tissue-type plasminogen activator gene polymorphisms with recurrent pregnancy loss in Iranian Azeri women.

The present case-control study was conducted in Azari women with RPL and healthy women during 2015-2016. Ninety-five women with RPL were selected by simple random sampling sampling method, and 95 healthy women were selected and assessed, using Multiplex ARMS-PCR method. The data were analyzed by SAS 9.2 software, using logistic regression and Chi-square test. Findings: Among two genes polymorphisms, only tPA polymorphism was significantly related with the increase in RPL, such that allele I in this polymorphism caused more than two-folded odds ratio of RPL compared to allele D. Women between the ages of 25 and 35 years significantly showed the highest odds ratio of RPL.

Alu_I/D tissue-type plasminogen activator gene polymorphism is related with an increased risk of RPL in Azeri women in northwestern Iran, but 536C/T tissue factor pathway inhibitor gene does not show such a relationship.