Evaluation of milk adenosine deaminase activities in dairy cattle with subclinical mastitis and their correlation with milk quality

The subclinical mastitis can cause economical loss, therefore, access to the methods of prevention, control and reduction of mastitis, especially subclinical form, is so important and valuable. The aim of this study was to investigate the changes of adenosine deaminase enzyme activity in the cow milk with subclinical mastitis. Forty-six cows with subclinical mastitis that have taken positive results in the California mastitis test (CMT), without signs of illness, were considered as the treatment group. Ten cows that have taken negative results in the CMT, were considered as milk samples control group. Fat and protein levels of milk were measured as well as somatic cells count. Adenosines deaminases enzyme activities were also measured. The comparison of fat and protein in milk samples demonstrated no significant difference between healthy group, and the one with subclinical mastitis. The number of somatic cells in the cows with subclinical mastitis was significantly more than the control group. In the cows with subclinical mastitis, adenosine deaminase enzyme activity was significantly higher than the healthy cows. In general, adenosine deaminase enzyme activity can be used as a marker in diagnosis of subclinical mastitis.