Evaluation of effect of linolenic acid on the quality of rooster semen stored at 4˚ C

The present study was designed to evaluate the effect of linolenic acid on the quality of rooster semen stored at 4˚ C. Semen was collected from ten roosters twice a week. Good quality ejaculates were pooled and then diluted, the semen was enriched with 0 (control), 40 (L40), 80 (L80) and 160 (L160) μM linoleate. Forward progressive motility (FPM), viability of spermatozoa, values of malondialdehyde (MDA) and total antioxidant capacity (TAC) in medium and spermatozoa were evaluated at 0, 24 and 48h. The result showed that, FPM was higher in L80 and L160 groups compared to control group at 24 and 48 h (P<0.05). Moreover, viability was greater in L80 and L160 groups in comparison with the control group at 48 h (P<0.05). Values of MDA were lower and values of TAC were greater in L80 and L160 groups in medium and spermatozoa compared to control group at 24 and 48 h (P<0.05). In conclusion, enrichment with of semen 80 and 160 μM linoleate could enhance quality of rooster semen stored at fridge temperature