Single Nucleotide Polymorphism in part of GH Gene and its Association with Carcass Traits in Afshari and Afshari-Booroola Merino sheep
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Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Growth hormone is one of the main factors affecting the growth of animals. There are different alleles of the gene leading different phenotypic effects. The aim of this study was to identify alleles of this gene in exon 5 and part of the 3 ‘UTR area. For this purpose, 133 lambs from a flock at almost the same age in three groups of pure Afshari breed and F2 and F5 crosses of Afshari- Booroola Merino were used. After taking Blood samples and recording the phenotypic measurements on live animals, 85 lambs were slaughtered. After slaughtering, carcass weight was measured and after 24 hours storing the carcasses in the cold room weight of thigh, shoulder, neck, fat tail and waste was measured. Regression coefficient Analyses were used to estimate carcass traits of those animals that not slaughtered. DNA was extracted by phenol-chloroform procedures from blood samples. Then, using primers designed, the desired fragment was amplified and directly sequenced. Afterward analyzing sequences, association of genotypes with carcass traits were evaluated. The results showed that in the nucleotide site of 2846 there were two genotypes, TT and TC, affecting the length of the body in pure Afshari breed and F5 crosses. In position 2882 of the gene sequence, a nucleotide was inserted in some animals and this insertion only in F2 crosses was associated with waste (carcass fat). The results show that the observed variations in case be proved in other evaluations can be used in marker assisted selection in sheep breeding programs.
Language:
Persian
Published:
Journal of Molecular and Cellular Research, Volume:31 Issue: 2, 2018
Pages:
232 to 242
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