Effect of different uranium ore concentration in the bioleaching process by analysis of Acidithiobacillus sp. FJ2 cytochrome A1 gene expression
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Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
The iron and sulfur oxidation process in Acidithiobacillus ferrooxidans is performed using an electron transport chain, which cycA1 as the most important component. Regarding the importance of this protein in the bioleaching process, the study of Acidithiobacillus ferrooxidans cycA1 gene in the presence of different pulp densities is the one of the main objectives to increase the efficiency of uranium bioleaching. For this purpose, the bacteria cultivated in the presence of different pulp densities of uranium ore (2.5, 5, 10 and 25%). Uranium extraction, variation of pH, Eh and ferrous iron values measured at 24 h intervals. Then, when the uranium extraction yield reached to 100%, gene expressions of cycA1 Acidithiobacillus ferrooxidans were analyzed. The results showed, with increasing pulp density, the uranium extraction rate and oxidation activity of bacteria was reduced. In addition, the results of cycA1 gene expression showed that the target gene expression increases in the presence of uranium ore compare to sample with absence of uranium ore, and with further increase of pulp density, due to the toxicity of uranium, show a decreasing trend. In addition, the changes of Eh, pH and uranium extractions at bioleaching process Acidithiobacillus have been delayed in the presence of high pulp density in compare with lower one. The results of cycA1 gene expressions bacteria in the presence of ore different concentrations showed that mutations and ore concentration has been effective on the expression of this gene.
Language:
Persian
Published:
Journal of Molecular and Cellular Research, Volume:31 Issue: 2, 2018
Pages:
203 to 220
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